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Registro Completo |
Biblioteca(s): |
Embrapa Florestas. |
Data corrente: |
20/07/2000 |
Data da última atualização: |
20/07/2000 |
Autoria: |
MARIMOM, B. S.; VARELLA, R. F.; MARIMOM JUNIOR, B. H. |
Título: |
Fitossociologia de uma area de cerrado de encosta em Nova Xavantina, Mato Grosso. |
Ano de publicação: |
1998 |
Fonte/Imprenta: |
Boletim do Herbario Ezechias Paulo Heringer, Brasilia, v.3, p.82-101. 1998. |
ISSN: |
0104-5334 |
Idioma: |
Português |
Palavras-Chave: |
Composicao floristica; Davilla elliptica; Fitossociologia; Foristic composition; Mato Grosso. |
Thesagro: |
Cerrado. |
Thesaurus Nal: |
plant ecology; savannas. |
Categoria do assunto: |
-- |
Marc: |
LEADER 00707naa a2200241 a 4500 001 1289272 005 2000-07-20 008 1998 bl uuuu u00u1 u #d 022 $a0104-5334 100 1 $aMARIMOM, B. S. 245 $aFitossociologia de uma area de cerrado de encosta em Nova Xavantina, Mato Grosso. 260 $c1998 650 $aplant ecology 650 $asavannas 650 $aCerrado 653 $aComposicao floristica 653 $aDavilla elliptica 653 $aFitossociologia 653 $aForistic composition 653 $aMato Grosso 700 1 $aVARELLA, R. F. 700 1 $aMARIMOM JUNIOR, B. H. 773 $tBoletim do Herbario Ezechias Paulo Heringer, Brasilia$gv.3, p.82-101. 1998.
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Embrapa Florestas (CNPF) |
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Registro Completo
Biblioteca(s): |
Embrapa Café. |
Data corrente: |
16/11/2022 |
Data da última atualização: |
16/11/2022 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
CASARIN, T.; FREITAS, N. C.; PINTO, R. T.; BREITLER, J. C.; RODRIGUES, L. A. Z.; MARRACCINI, P.; ETIENNE, H.; DINIZ, L. E. C.; ANDRADE, A. C.; PAIVA, L. V. |
Afiliação: |
TATIANE CASARIN, UNIVERSIDADE FEDERAL DE LAVRAS; NATÁLIA CHAGAS FREITAS, UNIVERSIDADE FEDERAL DE LAVRAS; RENAN TERASSI PINTO, UNIVERSIDADE FEDERAL DE LAVRAS; JEAN‐CHRISTOPHE BREITLER, CENTRO DE COOPERAÇÃO INTERNACIONAL EM PESQUISA AGRÍCOLA PARA O DESENVOLVIMENTO; LEONARDO AUGUSTO ZEBRAL RODRIGUES, UNIVERSIDADE FEDERAL DE LAVRAS; PIERRE MARRACCINI, CENTRO DE COOPERAÇÃO INTERNACIONAL EM PESQUISA AGRÍCOLA PARA O DESENVOLVIMENTO; HERVÉ ETIENNE, CENTRO DE COOPERAÇÃO INTERNACIONAL EM PESQUISA AGRÍCOLA PARA O DESENVOLVIMENTO; LEANDRO EUGENIO CARDAMONE DINIZ, CNPSO; ALAN CARVALHO ANDRADE, CNPCa; LUCIANO VILELA PAIVA, UNIVERSIDADE FEDERAL DE LAVRAS. |
Título: |
Multiplex CRISPR/Cas9-mediated knockout of the phytoene desaturase gene in Coffea canéfora. |
Ano de publicação: |
2022 |
Fonte/Imprenta: |
Scientific Reports, v. 12, 17270, 2022. 10 p. |
DOI: |
https://doi.org/10.1038/s41598-022-21566-w |
Idioma: |
Inglês |
Conteúdo: |
Coffea canephora (2n=2x22 chromosomes) is a species with extensive genetic diversity and desirable agronomic traits for coffee breeding programs. However, obtaining a new coffee cultivar through conventional breeding techniques may require more than 30 years of crossing cycles and selection, which hampers the effort of keeping up with market demands and rapidly proposing more resilient to climate change varieties. Although, the application of modern biotechnology tools such as precision genetic engineering technologies may enable a faster cultivar development process. Therefore, we aimed to validate the CRISPR/Cas9 system to generate mutations on a selected genotype of C. canephora, the clone 14. Embryogenic calli and a multiplex binary vector containing two sgRNAs targeting different exons of the CcPDS gene were used. The sgRNAs were under the C. canephora U6 promoter regulation. The target gene encodes phytoene desaturase, an enzyme essential for photosynthesis involved in B-carotene biosynthesis. Somatic seedlings and embryos with albino, variegated and green phenotypes regenerated after Agrobacterium tumefaciens-mediated genetic transformation were analyzed by verifying the insertion of the Cas9 gene and later by sequencing the sgRNAs target regions in the genome of Robusta modified seedlings. Among them, 77% had the expected mutations, and of which, 50% of them had at least one target with a homozygous mutation. The genotype, temperature of co-cultivation with the bacteria, and light intensity used for subsequent embryo regeneration appeared to strongly influence the successful regeneration of plants with a mutated CcPDS gene in the Coffea genus. MenosCoffea canephora (2n=2x22 chromosomes) is a species with extensive genetic diversity and desirable agronomic traits for coffee breeding programs. However, obtaining a new coffee cultivar through conventional breeding techniques may require more than 30 years of crossing cycles and selection, which hampers the effort of keeping up with market demands and rapidly proposing more resilient to climate change varieties. Although, the application of modern biotechnology tools such as precision genetic engineering technologies may enable a faster cultivar development process. Therefore, we aimed to validate the CRISPR/Cas9 system to generate mutations on a selected genotype of C. canephora, the clone 14. Embryogenic calli and a multiplex binary vector containing two sgRNAs targeting different exons of the CcPDS gene were used. The sgRNAs were under the C. canephora U6 promoter regulation. The target gene encodes phytoene desaturase, an enzyme essential for photosynthesis involved in B-carotene biosynthesis. Somatic seedlings and embryos with albino, variegated and green phenotypes regenerated after Agrobacterium tumefaciens-mediated genetic transformation were analyzed by verifying the insertion of the Cas9 gene and later by sequencing the sgRNAs target regions in the genome of Robusta modified seedlings. Among them, 77% had the expected mutations, and of which, 50% of them had at least one target with a homozygous mutation. The genotype, temperature of co-cultivation with the bacte... Mostrar Tudo |
Thesagro: |
Biodiversidade; Café Robusta; Coffea Canephora; Genótipo; Melhoramento Genético Vegetal; Mutação. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1148303/1/Multiplex-CRISPRCas9-mediated.pdf
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Marc: |
LEADER 02597naa a2200313 a 4500 001 2148303 005 2022-11-16 008 2022 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1038/s41598-022-21566-w$2DOI 100 1 $aCASARIN, T. 245 $aMultiplex CRISPR/Cas9-mediated knockout of the phytoene desaturase gene in Coffea canéfora.$h[electronic resource] 260 $c2022 520 $aCoffea canephora (2n=2x22 chromosomes) is a species with extensive genetic diversity and desirable agronomic traits for coffee breeding programs. However, obtaining a new coffee cultivar through conventional breeding techniques may require more than 30 years of crossing cycles and selection, which hampers the effort of keeping up with market demands and rapidly proposing more resilient to climate change varieties. Although, the application of modern biotechnology tools such as precision genetic engineering technologies may enable a faster cultivar development process. Therefore, we aimed to validate the CRISPR/Cas9 system to generate mutations on a selected genotype of C. canephora, the clone 14. Embryogenic calli and a multiplex binary vector containing two sgRNAs targeting different exons of the CcPDS gene were used. The sgRNAs were under the C. canephora U6 promoter regulation. The target gene encodes phytoene desaturase, an enzyme essential for photosynthesis involved in B-carotene biosynthesis. Somatic seedlings and embryos with albino, variegated and green phenotypes regenerated after Agrobacterium tumefaciens-mediated genetic transformation were analyzed by verifying the insertion of the Cas9 gene and later by sequencing the sgRNAs target regions in the genome of Robusta modified seedlings. Among them, 77% had the expected mutations, and of which, 50% of them had at least one target with a homozygous mutation. The genotype, temperature of co-cultivation with the bacteria, and light intensity used for subsequent embryo regeneration appeared to strongly influence the successful regeneration of plants with a mutated CcPDS gene in the Coffea genus. 650 $aBiodiversidade 650 $aCafé Robusta 650 $aCoffea Canephora 650 $aGenótipo 650 $aMelhoramento Genético Vegetal 650 $aMutação 700 1 $aFREITAS, N. C. 700 1 $aPINTO, R. T. 700 1 $aBREITLER, J. C. 700 1 $aRODRIGUES, L. A. Z. 700 1 $aMARRACCINI, P. 700 1 $aETIENNE, H. 700 1 $aDINIZ, L. E. C. 700 1 $aANDRADE, A. C. 700 1 $aPAIVA, L. V. 773 $tScientific Reports$gv. 12, 17270, 2022. 10 p.
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